HITRAP PROTEIN A HP
产品名称： HITRAP PROTEIN A HP
英文名称： HITRAP PROTEIN A HP
产品编号： 17-0402-01
产品价格： 0
产品产地： USA
品牌商标： 美国GE
更新时间： null
使用范围： null
上海基星生物科技有限公司
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983 点击查看
- 传真 : 点击查看
- 邮箱 : 342786402@qq.com
| 17-0402-01 | HITRAP PROTEIN A HP, 5 X 1 ML |
| 17-0402-03 | HITRAP PROTEIN A HP,2 X 1 ML |
| 17-0403-01 | HITRAP PROTEIN A HP,1 X 5 ML |
| 17-0403-03 | HITRAP PROTEIN A HP, 5 X 5 ML |
| 17-0404-01 | HITRAP PROTEIN G HP, 5 X 1 ML |
| 17-0404-03 | HITRAP PROTEIN G HP, 2 X 1 ML |
| 17-0405-01 | HITRAP PROTEIN G HP,1 X 5 ML |
| 17-0405-03 | HITRAP PROTEIN G HP, 5 X 5 ML |
| 17-0406-01 | HITRAP HEPARIN HP, 5 X 1 ML |
| 17-0407-01 | HITRAP HEPARIN HP,1 X 5 ML |
| 17-0407-03 | HiTrap Heparin HP 5x5ml |
| 17-0408-01 | HITRAP CHELATING HP, 5 X 1 ML |
| 17-0409-01 | HITRAP CHELATING HP, 1 X 5 ML |
| 17-0409-03 | HITRAP CHELATING HP 5 X 5 ML |
| 17-0409-05 | HITRAP CHELATING HP 100 X 5 ML |
| 17-0412-01 | HITRAP BLUE HP, 5 X 1 ML |
| 17-0413-01 | HITRAP BLUE HP, 1 X 5 ML |
| 17-0716-01 | HITRAP NHS-ACTIV HP, 5 X 1ML |
| 17-0716-30 | HiTrap NHS-activated HP, 180 x |
| 17-0717-01 | HITRAP NHS-ACTIV HP, 1 X 5 ML |
| 17-0920-03 | HITRAP IMAC HP, 5 X 1 ML |
| 17-0920-05 | HITRAP IMAC HP, 5 X 5 ML |
| 17-0921-02 | HITRAP IMAC FF, 5 X 1ML |
| 17-0921-04 | HITRAP IMAC FF, 5 X 5 ML |
| 17-0978-13 | HITRAP BUTYL-S FF, 5 X 1ML |
| 17-0978-14 | HITRAP BUTYL-S FF, 5 X 5ML |
HiTrap™ Protein A HP Columns
- Developed for purification and isolation of monoclonal and polyclonal IgG from ascites, serum, and cell culture supernatants.
- Prepacked with Protein A Sepharose™ High Performance for purification and fractionation of IgG subclasses. The coupling method gives high capacity, high performance, and low leakage levels.
- Fast binding kinetics.
- Binds 20 mg human IgG/ml medium.
- Stable over pH 3-9 for use, and 2-10 for cleaning.
HiTrap™ Protein A HP Columns
Technical Information
)
| TECHNICAL SPECIFICATIONS | |
| Ligand | Native protein A |
| Ligand coupling method | N-hydroxysuccinimide activation |
| Ligand density | » 3 mg protein A/ml medium |
| Matrix | Highly cross-linked agarose, 6% |
| Average particle size | 34 μm |
| Binding capacity | » 20 mg human IgG/ml medium |
| Recommended flow rate | 1 ml/min (1-ml column) 5 ml/min (5-ml column) |
| Maximum flow rate | 4 ml/min (1-ml column) 20 ml/min (5-ml column) |
| pH stability | 3-9 (long term), 2*-10 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 8°C |
| * pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
|
|
)
Purification of monoclonal mouse IgG2b on HiTrap™ Protein A HP